V-Labs, Inc. - Glycoconjugates

GLYCOPROTEINS

BLOOD GROUP & LEWIS ANTIGEN NEOGLYCOPROTEINS

CORE STRUCTURE NEOGLYCOPROTEINS

MONOSACCHARIDE NEOGLYCOPROTEINS

TUMOUR ANTIGEN NEOGLYCOPROTEINS

SIALYLATED NEOGLYCOPROTEINS

GALa1-3 GAL SERIES NEOGLYCOPROTEINS

GAL a1-3 GAL ANALOGUE NEOGLYCOPROTEINS

NEOGLYCOLIPIDS

Glycoconjugates

Dextra has developed unique techniques for coupling oligosaccharides to lipids and proteins with the aim of creating powerful tools for use in a range of applications. These include

Production of Monoclonal Antibodies

Using as little as 0.1 mg of neoglycoprotein per animal, is sufficient for the generation of antibodies to the required antigen. For this application, the short spacer arm version has been recommended by some of our customers.

While Dextra offers both Bovine Serum Albumin and Human Serum Albumin conjugates as standard catalogue items, a relatively inexpensive custom service is available for conjugate preparation to others such as Tetanus Toxoid or Keyhole Limpet Haemacyanin (KLH).

Special prices are available for bulk amounts or pyrogen-free material.

Enzyme Acceptors

Experience has shown that the use of Dextra's unique 14 atom spacer arm when using neoglycoproteins as glycosyl transferase acceptors has proved to be advantageous. As shown below, the ability of 14 atom spaced 3'SLN-BSA to act as an acceptor for a fucosyl transferase was comparable to the Bovine Fetuin control. Using the shorter spaced version, was essentially ineffective.

Dextra would therefore suggest that customers consider the steric influence of the protein prior to product selection.

ELISA

Many of our customers report that neoglycoproteins are ideal for ELISA.

Quality Assurance

All of Dextra's neoglycoproteins are analysed by Matrix Assisted Laser Desorption Time Of Flight mass spectrometry (MALDITOF). This permits the determination of the number of covalently added molecules of antigen per molecule of carrier protein, together with the molecular weight distribution (maximum and minimum numbers of residues per molecule).

The traces obtained for three conjugates (NGPs 0334, 0501, and 0502) are illustrated below. The molecular weight for BSA is 66330 daltons. Comparing the degree of peak broadening of the neoglycoprotein relative to the BSA, gives an indication of the heterodispersity of the conjugate. Generally, neoglycoproteins have an average of 10 to 12 carbohydrates per molecule of protein, with a range of between 7 or 8 to 18 or 20.

2 references relevant for ELISA test.
1. T.Cairns et al. Transplantation Vol 60, 1202-1207 1995
2. W. McKane et al. Transplantation Vol 66, 636-633 1998

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